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Western blotting was performed by standard immunoblotting procedure with 12% SDSPAGE gel, PVDF membrane, and primary antibodies against GPX4 (1:1,000, ab125066, Abcam), 4HNE (1 g ml 1 , MHN-20P, JaICA), human FSP1 (1:1,000, sc-377120, Santa Cruz), mouse FSP1 (1:100, clone 1A1 rat IgG2a

In other patients, symptoms become more severe and frequent over time

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10.1001/jamaneurol.2013.1274 12 ChavarraC.IvagnesR.SouzaJ

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